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Corning Life Sciences 384-well ultra-low attachment plates
384 Well Ultra Low Attachment Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Well Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Greiner Bio white plates
White Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences ultra-low attachment microtiter plates
Characterization of HNSCC MCTS morphology and viability in Ultra-low Attachment Plates. Eleven HNSCC cell lines and Het-1A cells were seeded at 2,500 cells per well in 384-well ULA-plates and cultured for 3 days. MCTSs were then stained with the live cell CAM and dead cell EHD reagents, and 4 × images were acquired on the IXM in the TL, FITC, and Texas Red channels. Grayscale TL images are presented along with color composite fluorescent images of live cell CAM and dead cell EHD staining depicted as green and red, respectively. Representative images from multiple independent experiments are presented. All scale bars represent 300 μm. CAM, Calcein AM; EHD, Ethidium Homodimer; HNSCC, head and neck squamous cell carcinoma; IXM, ImageXpress Micro; MCTS, multicellular tumor spheroid; TL, transmitted light; ULA-plates, ultra-low attachment <t>microtiter</t> plates.
Ultra Low Attachment Microtiter Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences low binding corning black round bottom 384-well plates
Characterization of HNSCC MCTS morphology and viability in Ultra-low Attachment Plates. Eleven HNSCC cell lines and Het-1A cells were seeded at 2,500 cells per well in 384-well ULA-plates and cultured for 3 days. MCTSs were then stained with the live cell CAM and dead cell EHD reagents, and 4 × images were acquired on the IXM in the TL, FITC, and Texas Red channels. Grayscale TL images are presented along with color composite fluorescent images of live cell CAM and dead cell EHD staining depicted as green and red, respectively. Representative images from multiple independent experiments are presented. All scale bars represent 300 μm. CAM, Calcein AM; EHD, Ethidium Homodimer; HNSCC, head and neck squamous cell carcinoma; IXM, ImageXpress Micro; MCTS, multicellular tumor spheroid; TL, transmitted light; ULA-plates, ultra-low attachment <t>microtiter</t> plates.
Low Binding Corning Black Round Bottom 384 Well Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences 384 well low-volume round bottom plate
Characterization of HNSCC MCTS morphology and viability in Ultra-low Attachment Plates. Eleven HNSCC cell lines and Het-1A cells were seeded at 2,500 cells per well in 384-well ULA-plates and cultured for 3 days. MCTSs were then stained with the live cell CAM and dead cell EHD reagents, and 4 × images were acquired on the IXM in the TL, FITC, and Texas Red channels. Grayscale TL images are presented along with color composite fluorescent images of live cell CAM and dead cell EHD staining depicted as green and red, respectively. Representative images from multiple independent experiments are presented. All scale bars represent 300 μm. CAM, Calcein AM; EHD, Ethidium Homodimer; HNSCC, head and neck squamous cell carcinoma; IXM, ImageXpress Micro; MCTS, multicellular tumor spheroid; TL, transmitted light; ULA-plates, ultra-low attachment <t>microtiter</t> plates.
384 Well Low Volume Round Bottom Plate, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences low-volume white microtiter plates
Characterization of HNSCC MCTS morphology and viability in Ultra-low Attachment Plates. Eleven HNSCC cell lines and Het-1A cells were seeded at 2,500 cells per well in 384-well ULA-plates and cultured for 3 days. MCTSs were then stained with the live cell CAM and dead cell EHD reagents, and 4 × images were acquired on the IXM in the TL, FITC, and Texas Red channels. Grayscale TL images are presented along with color composite fluorescent images of live cell CAM and dead cell EHD staining depicted as green and red, respectively. Representative images from multiple independent experiments are presented. All scale bars represent 300 μm. CAM, Calcein AM; EHD, Ethidium Homodimer; HNSCC, head and neck squamous cell carcinoma; IXM, ImageXpress Micro; MCTS, multicellular tumor spheroid; TL, transmitted light; ULA-plates, ultra-low attachment <t>microtiter</t> plates.
Low Volume White Microtiter Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences peptide substrate to 384 low volume plate
Characterization of HNSCC MCTS morphology and viability in Ultra-low Attachment Plates. Eleven HNSCC cell lines and Het-1A cells were seeded at 2,500 cells per well in 384-well ULA-plates and cultured for 3 days. MCTSs were then stained with the live cell CAM and dead cell EHD reagents, and 4 × images were acquired on the IXM in the TL, FITC, and Texas Red channels. Grayscale TL images are presented along with color composite fluorescent images of live cell CAM and dead cell EHD staining depicted as green and red, respectively. Representative images from multiple independent experiments are presented. All scale bars represent 300 μm. CAM, Calcein AM; EHD, Ethidium Homodimer; HNSCC, head and neck squamous cell carcinoma; IXM, ImageXpress Micro; MCTS, multicellular tumor spheroid; TL, transmitted light; ULA-plates, ultra-low attachment <t>microtiter</t> plates.
Peptide Substrate To 384 Low Volume Plate, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences low volume 384-well black wall clear bottom tissue culture plate
Characterization of HNSCC MCTS morphology and viability in Ultra-low Attachment Plates. Eleven HNSCC cell lines and Het-1A cells were seeded at 2,500 cells per well in 384-well ULA-plates and cultured for 3 days. MCTSs were then stained with the live cell CAM and dead cell EHD reagents, and 4 × images were acquired on the IXM in the TL, FITC, and Texas Red channels. Grayscale TL images are presented along with color composite fluorescent images of live cell CAM and dead cell EHD staining depicted as green and red, respectively. Representative images from multiple independent experiments are presented. All scale bars represent 300 μm. CAM, Calcein AM; EHD, Ethidium Homodimer; HNSCC, head and neck squamous cell carcinoma; IXM, ImageXpress Micro; MCTS, multicellular tumor spheroid; TL, transmitted light; ULA-plates, ultra-low attachment <t>microtiter</t> plates.
Low Volume 384 Well Black Wall Clear Bottom Tissue Culture Plate, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences 384-well low volume corning nbs plate
Characterization of HNSCC MCTS morphology and viability in Ultra-low Attachment Plates. Eleven HNSCC cell lines and Het-1A cells were seeded at 2,500 cells per well in 384-well ULA-plates and cultured for 3 days. MCTSs were then stained with the live cell CAM and dead cell EHD reagents, and 4 × images were acquired on the IXM in the TL, FITC, and Texas Red channels. Grayscale TL images are presented along with color composite fluorescent images of live cell CAM and dead cell EHD staining depicted as green and red, respectively. Representative images from multiple independent experiments are presented. All scale bars represent 300 μm. CAM, Calcein AM; EHD, Ethidium Homodimer; HNSCC, head and neck squamous cell carcinoma; IXM, ImageXpress Micro; MCTS, multicellular tumor spheroid; TL, transmitted light; ULA-plates, ultra-low attachment <t>microtiter</t> plates.
384 Well Low Volume Corning Nbs Plate, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cayman Chemical 384-well solid plate low volume black
Characterization of HNSCC MCTS morphology and viability in Ultra-low Attachment Plates. Eleven HNSCC cell lines and Het-1A cells were seeded at 2,500 cells per well in 384-well ULA-plates and cultured for 3 days. MCTSs were then stained with the live cell CAM and dead cell EHD reagents, and 4 × images were acquired on the IXM in the TL, FITC, and Texas Red channels. Grayscale TL images are presented along with color composite fluorescent images of live cell CAM and dead cell EHD staining depicted as green and red, respectively. Representative images from multiple independent experiments are presented. All scale bars represent 300 μm. CAM, Calcein AM; EHD, Ethidium Homodimer; HNSCC, head and neck squamous cell carcinoma; IXM, ImageXpress Micro; MCTS, multicellular tumor spheroid; TL, transmitted light; ULA-plates, ultra-low attachment <t>microtiter</t> plates.
384 Well Solid Plate Low Volume Black, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences 384 well assay plate corning®, low volume, non-binding surface
Characterization of HNSCC MCTS morphology and viability in Ultra-low Attachment Plates. Eleven HNSCC cell lines and Het-1A cells were seeded at 2,500 cells per well in 384-well ULA-plates and cultured for 3 days. MCTSs were then stained with the live cell CAM and dead cell EHD reagents, and 4 × images were acquired on the IXM in the TL, FITC, and Texas Red channels. Grayscale TL images are presented along with color composite fluorescent images of live cell CAM and dead cell EHD staining depicted as green and red, respectively. Representative images from multiple independent experiments are presented. All scale bars represent 300 μm. CAM, Calcein AM; EHD, Ethidium Homodimer; HNSCC, head and neck squamous cell carcinoma; IXM, ImageXpress Micro; MCTS, multicellular tumor spheroid; TL, transmitted light; ULA-plates, ultra-low attachment <t>microtiter</t> plates.
384 Well Assay Plate Corning®, Low Volume, Non Binding Surface, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Characterization of HNSCC MCTS morphology and viability in Ultra-low Attachment Plates. Eleven HNSCC cell lines and Het-1A cells were seeded at 2,500 cells per well in 384-well ULA-plates and cultured for 3 days. MCTSs were then stained with the live cell CAM and dead cell EHD reagents, and 4 × images were acquired on the IXM in the TL, FITC, and Texas Red channels. Grayscale TL images are presented along with color composite fluorescent images of live cell CAM and dead cell EHD staining depicted as green and red, respectively. Representative images from multiple independent experiments are presented. All scale bars represent 300 μm. CAM, Calcein AM; EHD, Ethidium Homodimer; HNSCC, head and neck squamous cell carcinoma; IXM, ImageXpress Micro; MCTS, multicellular tumor spheroid; TL, transmitted light; ULA-plates, ultra-low attachment microtiter plates.

Journal: Assay and Drug Development Technologies

Article Title: High Content Screening Characterization of Head and Neck Squamous Cell Carcinoma Multicellular Tumor Spheroid Cultures Generated in 384-Well Ultra-Low Attachment Plates to Screen for Better Cancer Drug Leads

doi: 10.1089/adt.2018.896

Figure Lengend Snippet: Characterization of HNSCC MCTS morphology and viability in Ultra-low Attachment Plates. Eleven HNSCC cell lines and Het-1A cells were seeded at 2,500 cells per well in 384-well ULA-plates and cultured for 3 days. MCTSs were then stained with the live cell CAM and dead cell EHD reagents, and 4 × images were acquired on the IXM in the TL, FITC, and Texas Red channels. Grayscale TL images are presented along with color composite fluorescent images of live cell CAM and dead cell EHD staining depicted as green and red, respectively. Representative images from multiple independent experiments are presented. All scale bars represent 300 μm. CAM, Calcein AM; EHD, Ethidium Homodimer; HNSCC, head and neck squamous cell carcinoma; IXM, ImageXpress Micro; MCTS, multicellular tumor spheroid; TL, transmitted light; ULA-plates, ultra-low attachment microtiter plates.

Article Snippet: Generation of HNSCC MCTSs in Ultra-Low Attachment Microtiter Plates We have previously described the generation of MCTSs after seeding several HNSCC cell lines into 384-well U-bottomed ultra-low attachment microtiter plates (ULA-plates; Cat. No. 4516; Corning, Tewksbury, MA).

Techniques: Cell Culture, Staining